| Buffalo Bill Museum and Lone Star Steamer |
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| Des Moines County Heritage Museum |
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| Driftless Area Education and Visitor Center |
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| EB Lyons Interpretive Center at Mines of Spain |
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| Effigy Mounds National Monument |
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| George M. Verity Riverboat Museum |
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| Hurstville Interpretive Center |
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| Iowa Department of Natural Resources Fish Aquarium |
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| Motor Mill Historic Site |
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| Nahant Marsh Education Center |
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| National Mississippi River Museum & Aquarium |
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| Old Fort Madison |
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| Pine Creek Grist Mill |
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| Putnam Museum and Science Center |
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| The National Pearl Button Museum |
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| The Sawmill Museum |
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| Toolesboro Mounds National Historic Landmark |
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